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WebApr 6, 2024 · We performed 16 cycles for the short-read index PCR and 12 cycles for the long-read template generation. We used FLT-seq as this protocol generates a high proportion of full-length (3′ adaptor to 5′ TSO) reads and an almost negligible proportion of TSO artifacts (TSO-TSO reads without a valid cell barcode). Short-read Illumina sequencing WebFigure Legend Snippet: ( a ) Candidate TSO sequences and previous TSO (“Smartseq3 Original”) were evaluated in human PBMC samples with a change to 0.125 µM oligo-dT primer. At indicated sequencing depths, we show the median number of detected genes (left), median number of detected genes after discarding TSO-priming artifact reads … Web3. TSO artifact removal In this step, removal of DNA fragments containing TSO artifacts is performed using MAS capture beads. Step Instructions 3.1 Bring MAS capture bead kit to room temperature. Resuspend the beads by vortexing. 3.2 Transfer 10 μL resuspended MAS capture beads to a PCR tube. of beads. grant for trucking business