Nepenthesin-1
WebNepenthesin-1. 2.1 mm ID x 20 mm length, Cat.No.: AP-PC-003 (volume: 69.3 µL, 10-32 fitting for 1/16″ OD tubing) 1.0 mm ID x 20 mm length, Cat.No.: AP-PC-003s (volume: … WebJan 5, 2024 · The carnivorous pitcher plant Nepenthes alata produces digestive enzymes including proteases which degrade proteins in captured insect prey. Nepenthesin I (NEP1) is an aspartic protease similar to pepsin that is found in human stomachs. Nepenthesin is unique in that it cleaves on either side of an aspartate residue and is extremely stable in …
Nepenthesin-1
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WebMar 1, 2014 · The presence of nepenthesin-1 gene without the plant signal sequence in pBSSK − was confirmed by restriction digestion analysis and DNA sequencing. The … WebA nepenthesin 1-type insert and a helix–turn–helix domain feature prominently at opposite surfaces of the protein. The nepenthesin insert—so-called as it was identified in the protease nepenthesin 1 from the digestive fluid of the pitcher plant Nepenthesia—is a trilobed structure held together by two pairs of disulfide-bonded cysteines (11.
WebJul 5, 2004 · UniProtKB reviewed (Swiss-Prot) Organism. Nepenthes gracilis (Slender pitcher plant) Amino acids. 437. Protein existence. Evidence at protein level. Annotation … Web0433389 - ÚMCH 2015 DK eng A - Abstract Fejfarová, Karla - Man, Petr - Mrázek, Hynek - Kádek, Alan - Halada, Petr - Skálová, Tereza - Kolenko, Petr - Švecová, L. - Hašek, …
WebJan 1, 2016 · They significantly differ in sequence from other plant aspartic proteases. This difference, which provides more cysteine residues in the structure of nepenthesins, may … WebFeb 5, 2024 · Figure S1: Peptide map showing the sequence coverage of Nc LPMO9C after serial nepenthesin-1 / rhizopuspepsin digestion, Figure S2: Nc LPMO9C structural changes induced by the temperature, Figure S3: Selected examples of deuterium uptake curves for holo-/apo- Nc LPMO9C after temperature correction, Figure S4: Destabilization of the Nc …
WebPepsin, Rhisopuspepsin, Nepenthesin-1, Nepenthesin-2 and Aspergillopepsin. The selection of protease column or their combination together with smart choice of …
WebSep 1, 2024 · A nepenthesin 1-type insert and a helix–turn–helix domain feature prominently at opposite surfaces of the protein. The nepenthesin insert—so-called as it … poems about dictatorshipWebPetr Man 1 , 2 show more details. Series: Methods In Molecular Biology > Book: Heterologous Expression of Membrane Proteins. Protocol DOI: 10.1007/978-1-4939-3637-3_17. Affiliations: BioCeV - Institute of Microbiology, Czech Academy of Sciences, Vestec, Czech Republic; Faculty of Science ... poems about death poetry foundationWebApr 26, 2024 · nepenthesin ( Nepenthes gracilis ), Uniprot accession Q766C3 (peptidase unit: 75-437), MERNUM MER0031323. History. Identifier created: MEROPS 4.0 (27 … poems about death of a sisterWebJun 20, 2016 · Phytohormone levels were determined by LC-MS/MS. Nepenthesin proteolytic activities were measured in the digestive fluid using a fluorescence substrate. … poems about destiny and fateWebSolution-phase hydrogen/deuterium exchange (HDX) monitored by high-resolution Fourier transform ion cyclotron resonance (FTICR) mass spectrometry offers a rapid method to study protein conformations and protein−protein interactions. Pepsin is usually used to digest proteins in HDX and is known for lack of cleavage specificity. To improve digestion … poems about deer and treesWebApr 12, 2024 · However, even the highly denaturation-prone protease nepenthesin-1 can withstand harsh denaturing conditions if used in immobilized form [27, 41]. The … poems about death of sonWebPlease reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term … poems about difficult times