Irf8 flox mouse
WebCreLysMFasflox/flox mice expressed Fas on B and T lym-phocytes isolated from peripheral blood (ref. 10 and data not shown) and the peritoneal cavity (Figure 1A). In contrast, Fas … WebThe Irf8 floxed targeted mutated mouse strain can be used to selectively ablate expression of IRF8 in any cell type in which a Cre recombinase gene is activated. This will permit the...
Irf8 flox mouse
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WebDuring our analyses, we also examined littermate mice with a genotype of floxed IRF8 only, PU.1 only, or IRF8/PU.1 heterozygous with or without Mb1-Cre (SI Appendix, Table S1). While Mb1-Cre–mediated PU.1 deletion alone did not affect B cell distribution in the BM, Mb1-Cre–mediated IRF8 deletion alone appeared to induce a moderate reduction ... Web014175 B6.Cg- Irf8 tm1.1Hm /J This Irf8 f floxed targeted mutation strain may be useful in generating conditional mutants for studying B cell differentiation, immunodeficiency …
WebMyeloid cell-specific IRF8 conditional knockout mice (IRF8flox/flox; Lyz2Cre/+) with a C57BL/6 background were generated by mating IRF8-flox mice (Feng et al. 2011) with Lyz2-Cre knock-in mice. The primers used for genotyping are shown in Supplementary Table S3. The genotyping results are shown in Supplementary Figure S4a. Harvesting of blood ... WebFeb 11, 2024 · Mechanistically, loss of SENP3 increases interferon regulatory factor 8 (IRF8) SUMO3 modification at the K310 amino acid site, which upregulates expression of the nuclear factor of activated T cell c1 (NFATc1) and osteoclastogenesis.
WebIRF8 and IRF1 are transcriptional regulators that play critical roles in the development and function of myeloid cells, including activation of macrophages by proinflammatory signals … WebDec 11, 2024 · Mice with IRF8 deficiency only in colon epithelial cells (termed IRF8cKO) were created by crossing Irf8-floxed mice with villin-cre mice (Figures S1A and S1B) with the deletion of exon 2 (Figure S1C). As reported in the literature, we observed that IRF8KO mice exhibit elevated levels of CD11b + Gr1 + MDSCs as compared to wild-type (WT) mice. …
WebMar 8, 2024 · SSc remain unknown. IRF8 is a transcriptional regulator that is essential for the differentiation and function of monocytes and macrophages and thus may be involved in the regulation of macrophage phenotypes in SSc fibrosis. In this study, we measured IRF8 levels in circulating monocytes of 26 patients with SSc (diffuse cutaneous SSc,
WebJan 1, 2014 · Here we show that IRF8 is downregulated in both mouse hearts and cardiomyocytes after hypertrophic stress treatment. Moreover, IRF8-deficient mice reveal an aggravated pressure overload-induced hypertrophic response, whereas the opposite effects occur in IRF8-overexpressing mice. notes on crpc pdfWebCopies of IRF8 flox or delta locus/ reference gene locus CD45.2 (normalized) ... Mononuclear phagocyte composition in Itgax-cre.Irf8fl/fl or fl/- and Irf8fl/fl mice and effect of Irf8 haplo-insufficiency on intestinal DC subset composition, related to Figure 1. (A and C) Representative flow cytometry plots and (B and D) ... how to set up a club bank accountWebInterferon regulatory factor 8 (Irf8), also known as interferon consensus sequence-binding protein, is a component of the Irf transcription factor family. Irf8 is differentially expressed … notes on cpuWebFeb 14, 2014 · Here we show that IRF8 is downregulated in both mouse hearts and cardiomyocytes after hypertrophic stress treatment. Moreover, IRF8-deficient mice reveal an aggravated pressure... notes on credit cardsWebJun 21, 2024 · We generated a mouse line with Irf8 -deficiency in Clec9a -expressing progenitors and hypothesized that Irf8 -deficiency would deplete cDC1s, which may promote a pro-inflammatory immune response and consequently drive AKI/AKD progression. Materials and Methods Animals how to set up a clynk accountnotes on criminal lawWebMar 8, 2024 · Because IRF8 may contribute to SSc progression, including fibrosis, vascular abnormalities, and impaired wound healing, we evaluated IRF8 levels in SSc monocytes … how to set up a coffee station at home